Israeli troops shot dead two Palestinian protesters along the Gaza border on Friday, Palestinian medical officials in Gaza said, raising the death toll to 22 in the week-long disturbances. They said the men were killed at protest sites east of Gaza City and Khan Younis during a round of daily demonstrations that began on March 30 and are known as “The Great March of Return”. Gazas health ministry also reported that around 150 Palestinians were wounded by the renewed clashes. Palestinians burned mounds of tires and threw stones at Israeli soldiers over the border fence, who responded with tear gas and live fire. Thousands of protesters gathered in locations near the border east of Khan Yunis, in the south of the blockaded Palestinian enclave, and east of Gaza City. The number of protesters was however much lower than last week, when a demonstration by tens of thousands led to clashes in which Israeli forces killed 19 Palestinians. The protesters, including Palestinian refugees and their descendants seeking to regain homes in what is now Israel that were lost during its 1948-49 independence war, have set up tent encampments a few hundred meters (yards) inside Gaza. Large groups of youths have ventured much closer to the no-go zone along the border, risking live fire from Israeli troops to roll burning tires at the barrier and throw stones. The tire fires were meant to be a smokescreen from Israeli snipers, and thick black smoke covered the border area in some places. Israeli forces took up positions across a ridge on the other side of the border and set up large fans in an apparent bid to push away the smoke. They also used water cannon to try to douse some of the flames. "I will be a martyr today. I will cross the border," Ahmed Abu Ghali, 20, who held up his shirt to show his still seeping wound from last week that required 40 stitches. "I was wounded last Friday but escaped yesterday from hospital," said the protester at the rally east of Khan Yunis. Israels military said there had been "rioting" in five locations along the border. It added that "troops are responding with riot dispersal means, and fire in accordance with the rules of engagement". Israel had warned that its open-fire rules would remain unchanged for Fridays protests, pledging to stop any damage to the fence and prevent infiltrations or alleged attacks. But it has faced mounting criticism over its use of live fire, and UN chief Antonio Guterres called for restraint. "I particularly urge Israel to exercise extreme caution with the use of force in order to avoid casualties. Civilians must be able to exercise their right to demonstrate peacefully," he said in a statement. - Calculated - In last Fridays demonstration, a number strayed from the main protest and approached the heavily fortified fence on the border with Israel. Israel says troops opened fire only when necessary against those throwing stones and firebombs or rolling burning tyres at soldiers. It said there were attempts to damage the fence and infiltrate Israel, while alleging there was also an attempted gun attack against soldiers along the border. It accuses Hamas, the Islamist movement that runs the Gaza Strip and with whom it has fought three wars since 2008, of using the protests as cover to carry out violence. But the toll of those killed and wounded on March 30 -- more than 750 people were injured by gunfire, according to Gazas health ministry -- has led to criticism of Israel. There were no Israeli casualties. Palestinians say protesters were shot while posing no threat to soldiers, and unverified videos that have spread online have fuelled their accusations. The videos include one appearing to show a man with a tire shot while running away from the fence. Human Rights Watch has called the actions by the Israeli soldiers "calculated" and illegal. Guterres and the European Union have called for an independent investigation, which Israel has outright rejected. The protests, designed to last six weeks, are in support of refugees, including those in the Palestinian enclave who want to return to their former homes in what is now Israel. More than 700,000 Palestinians fled or were expelled from their lands during the war surrounding Israels creation in 1948. Israelis say allowing the so-called "right of return" would mean their country would cease to exist. The White House on Thursday refrained from criticism of Israel and called on protesters to remain peaceful. President Donald Trumps envoy Jason Greenblatt said protesters "should remain outside the 500-metre (-yard) buffer zone; and should not approach the border fence in any way or any location".