Gillingham provided the biggest shock of the Carabao Cup first round by knocking out last season’s semi-finalists Southampton. Ashley Nadesan’s goal and a Robbie McKenzie double saw the League Two side beat the team just relegated from the Premier League 3-1 at Priestfield. The Southampton manager, Russell Martin, made 11 changes from the side that won at Sheffield Wednesday on Friday, but it backfired spectacularly despite a late consolation from the substitute Carlos Alcaraz. Hull were also Championship casualties, beaten 2-1 by League Two Doncaster thanks to two goals from George Miller after Óscar Estupiñán had given Hull the lead. Millwall joined them through the exit after two goals from Kelvin Ehibhatiomhan and further strikes from Charlie Savage and Mamadi Camara gave troubled Reading a thumping 4-0 win at the Den. Three Championship sides were dumped out on penalties by lower-division clubs. Sunderland lost the shootout 5-3 at Crewe after a 1-1 draw, Stevenage held their nerve to knock out Watford and Salford took the scalp of Preston. Sheffield Wednesday needed a Tyreeq Bakinson equaliser seven minutes into stoppage time to earn a 1-1 draw with Stockport, with Wednesday winning the shootout 4-1. Aiden Marsh left it even later, equalising for Barnsley in the 98th minute to earn a 2-2 draw with Tranmere, but League Two Rovers triumphed 7-6 on spot-kicks. League Two side Newport fought back from a goal down to beat League One Charlton 3-1. Newly promoted Wrexham, playing their first League Cup tie since 2007, went through on penalties after a goalless draw at home to Wigan. Peterborough won on spot-kicks after a 1-1 draw with Swindon, as did Rotherham, who drew at home to Morecambe. Sutton were also victorious from 12 yards after a 2-2 draw with Cambridge, and Bradford saw off League Two rivals Accrington 4-1 on penalties following a 1-1 draw. Stoke beat West Brom 2-1 and Middlesbrough won the other all-Championship clash at Huddersfield 3-2. Joël Piroe struck twice to give new Swansea manager Michael Duff his first win, 3-0 at home to Northampton, and two goals from Juninho Bacuna saw Birmingham through with a 2-0 success at Cheltenham. There were also wins for Blackpool, Bolton, Plymouth, Port Vale, Portsmouth, Exeter, Mansfield, Lincoln, Wycombe and Harrogate.